%0 Journal Article
	%A K. Ratanapongleka and  J. Phetsom
	%D 2011
	%J International Journal of Chemical and Molecular Engineering
	%B World Academy of Science, Engineering and Technology
	%I Open Science Index 57, 2011
	%T Extraction in Two-Phase Systems and Some Properties of Laccase from Lentinus polychrous
	%U https://publications.waset.org/pdf/11632
	%V 57
	%X Extraction of laccase produced by L. polychrous in an
aqueous two-phase system, composed of polyethylene glycol and
phosphate salt at pH 7.0 and 250C was investigated. The effect of
PEG molecular weight, PEG concentration and phosphate
concentration was determined. Laccase preferentially partitioned to
the top phase. Good extraction of laccase to the top phase was
observed with PEG 4000. The optimum system was found in the
system containing 12% w/w PEG 4000 and 16% w/w phosphate salt
with KE of 88.3, purification factor of 3.0-fold and 99.1% yield.
Some properties of the enzyme such as thermal stability, effect of
heavy metal ions and kinetic constants were also presented in this
work. The thermal stability decreased sharply with high temperature
above 60 0C. The enzyme was inhibited by Cd2+, Pb2+, Zn2+ and
Cu2+. The Vmax and Km values of the enzyme were 74.70
μmol/min/ml and 9.066 mM respectively.
	%P 822 - 825