%0 Journal Article %A Congmin Z. Xie and Terry Healy and Peter Robinson and Kevin Stewart %D 2008 %J International Journal of Environmental and Ecological Engineering %B World Academy of Science, Engineering and Technology %I Open Science Index 20, 2008 %T Determination of EDTA in Dairy Wastewater and Adjacent Surface Water %U https://publications.waset.org/pdf/10699 %V 20 %X An HPLC-UV analytical method was developed to determine ethylenediaminetetraacetic acid (EDTA) in dairy wastewater and surface water. The optimizing separation was achieved by reversed–phase ion-pair liquid chromatography on a C18 column using methanol as mobile phase solvent, tetrabutylammonium bromide as the ion-pair reagent in pH 3.3 formate buffer solution at a flow rate of 0.9 mL min-1 with a UV detector at 265 nm. No interference of Ca, Mg or NO3 - was detected. Method performance was evaluated in terms of linearity, repeatability and reproducibility. The method detection limit was 5 μg L-1. The contents of EDTA in dairy effluents were 72 ~ 261 μg L-1 at a large dairy site. A change of EDTA concentration was observed downstream of the dairy effluent discharge, but this was well under the predicted no effect concentration for aquatic ecosystem. %P 82 - 86