TY - JFULL AU - N. Hussain and G. Jaffery and A.N. Sabri and S. Hasnain PY - 2011/7/ TI - Mutational Analysis of CTLA4 Gene in Pakistani SLE Patients T2 - International Journal of Bioengineering and Life Sciences SP - 246 EP - 249 VL - 5 SN - 1307-6892 UR - https://publications.waset.org/pdf/10037 PU - World Academy of Science, Engineering and Technology NX - Open Science Index 54, 2011 N2 - The main aim is to perform mutational analysis of CTLA4 gene Exon 1 in SLE patients. A total of 61 SLE patients fulfilling “American College of Rheumatology (ACR) criteria" and 61 controls were enrolled in this study. The region of CTLA4 gene exon 1 was amplified by using Step-down PCR technique. Extracted DNA of band 354 bp was sequenced to analyze mutations in the exon-1 of CTLA-4 gene. Further, protein sequences were identified from nucleotide sequences of CTLA4 Exon 1 by using Expasy software and through Blast P software it was found that CTLA4 protein sequences of Pakistani SLE patients were similar to that of Chinese SLE population. No variations were found after patients sequences were compared with that of the control sequence. Furthermore it was found that CTLA4 protein sequences of Pakistani SLE patients were similar to that of Chinese SLE population. Thus CTLA4 gene may not be responsible for an autoimmune disease SLE. ER -