Elhamalsadat Ghaffari and Moghan Amirhosseinian and Amir Khaleghipour
Investigating the Formation of NanoHydroxyapatite on a Biocompatible and Antibacterial CuMgSubstituted Bioglass
339 - 348
2019
13
7
International Journal of Chemical and Materials Engineering
https://publications.waset.org/pdf/10010574
https://publications.waset.org/vol/151
World Academy of Science, Engineering and Technology
Multifunctional bioactive glasses (BGs) are designed with a focus on the provision of bactericidal and biological properties desired for angiogenesis, osteogenesis, and ultimately potential applications in bone tissue engineering. To achieve these, six solgel coppermagnesium substituted derivatives of 58SBG, i.e. a mol series of 60SiO24P2O55CuO(31x) CaOxMgO (where x0, 1, 3, 5, 8, and 10), were synthesized. Afterwards, the effect of MgOCaO substitution on the in vitro formation of nanohydroxyapatite (HA), osteoblastlike cell responses and BGs antibacterial performance were studied. During the BGs synthesis, the elimination of nitrates was achieved at 700 °C that prevented the BGs crystallization and stabilized the obtained dried gels. The structural and morphological evaluations were performed with Xray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). These characterizations revealed that Cusubstituted 58SBG consisting of 5 mol MgO (BG55) slightly had retarded the formation of HA. In addition, Cusubstituted 58SBGs consisting 8 mol and 10 mol MgO (BG58 and BG510) displayed lower bioactivity probably due to the lower ion release rate of Ca–Si into the simulated body fluid (SBF). The determination of 3(4, 5 dimethylthiazol2yl)2, 5diphenyltetrazolium bromide (MTT) and alkaline phosphate (ALP) activities proved that the highest values of both differentiation and proliferation of MC3T3E1 cells can be obtained from a 5 mol MgO substituted BG, while the over addition of MgO (8 mol and 10 mol) decreased the bioactivity. Furthermore, these novel CuMgsubstituted 58SBGs displayed antibacterial effect against methicillinresistant Staphylococcus aureus bacteria. Taken together, the results suggest the equallysubstituted BG55 (i.e. the one consists of 5 mol of both CuO and MgO) as a promising candidate for bone tissue engineering, among all newly designed BGs in this work, owing to its desirable cell proliferation, ALP activity and antibacterial properties.
Open Science Index 151, 2019