@article{(Open Science Index):https://publications.waset.org/pdf/10008830, title = {miR-200c as a Biomarker for 5-FU Chemosensitivity in Colorectal Cancer}, author = {Rezvan Najafi and Korosh Heydari and Massoud Saidijam }, country = {}, institution = {}, abstract = {5-FU is a chemotherapeutic agent that has been used in colorectal cancer (CRC) treatment. However, it is usually associated with the acquired resistance, which decreases the therapeutic effects of 5-FU. miR-200c is involved in chemotherapeutic drug resistance, but its mechanism is not fully understood. In this study, the effect of inhibition of miR-200c in sensitivity of HCT-116 CRC cells to 5-FU was evaluated. HCT-116 cells were transfected with LNA-anti- miR-200c for 48 h. mRNA expression of miR-200c was evaluated using quantitative real- time PCR. The protein expression of phosphatase and tensin homolog (PTEN) and E-cadherin were analyzed by western blotting. Annexin V and propidium iodide staining assay were applied for apoptosis detection. The caspase-3 activation was evaluated by an enzymatic assay. The results showed LNA-anti-miR-200c inhibited the expression of PTEN and E-cadherin protein, apoptosis and activation of caspase 3 compared with control cells. In conclusion, these results suggest that miR-200c as a prognostic marker can overcome to 5-FU chemoresistance in CRC. }, journal = {International Journal of Medical and Health Sciences}, volume = {12}, number = {2}, year = {2018}, pages = {13 - 18}, ee = {https://publications.waset.org/pdf/10008830}, url = {https://publications.waset.org/vol/134}, bibsource = {https://publications.waset.org/}, issn = {eISSN: 1307-6892}, publisher = {World Academy of Science, Engineering and Technology}, index = {Open Science Index 134, 2018}, }