Bahdja Guerfi and N. Hadhoum and I. Azouz and M. Bendoumia and S. Bouafia and F. Z. Hadjadj Aoul
Characterization of Penicillin V Acid and Its Related Compounds by HPLC
846 - 850
2017
11
12
International Journal of Chemical and Molecular Engineering
https://publications.waset.org/pdf/10008454
https://publications.waset.org/vol/132
World Academy of Science, Engineering and Technology
Background &39;Penicillin V&39; is a narrow, bactericidal antibiotic of the betalactam family of the naturally occurring penicillin group. It is limited to infections due to the germs defined as sensitive. The objective of this work was to identify and to characterize Penicillin V acid and its related compounds by Highperformance liquid chromatography (HPLC). Methods Firstly phenoxymethylpenicillin was identified by an infrared absorption. The organoleptic characteristics, pH, and determination of water content were also studied. The dosage of Penicillin V acid active substance and the determination of its related compounds were carried on waters HPLC, equipped with a UV detector at 254 nm and Discovery HS C18 column (250 mm X 4.6 mm X 5 µm) which is maintained at room temperature. The flow rate was about 1 ml per min. A mixture of water, acetonitrile and acetic acid (653501) was used as mobile phase for phenoxyacetic acid ‘impurity B&39; and a mixture of water, acetonitrile and acetic acid (6501505.75) for the assay and 4hydroxypenicillin V &39;impurity D&39;. Results The identification of Penicillin V acid active substance and the evaluation of its chemical quality showed conformity with USP 35th edition. The Penicillin V acid content in the raw material is equal to 1692.22 UImg. The percentage content of phenoxyacetic acid and 4hydroxypenicillin V was respectively 0.035 and 0.323. Conclusion Through these results, we can conclude that the Penicillin V acid active substance tested is of good physicochemical quality.
Open Science Index 132, 2017