@article{(Open Science Index):https://publications.waset.org/pdf/10008143, title = {Microfluidic Paper-Based Electrochemical Biosensor}, author = {Ahmad Manbohi and Seyyed Hamid Ahmadi }, country = {}, institution = {}, abstract = {A low-cost paper-based microfluidic device (PAD) for the multiplex electrochemical determination of glucose, uric acid, and dopamine in biological fluids was developed. Using wax printing, PAD containing a central zone, six channels, and six detection zones was fabricated, and the electrodes were printed on detection zones using pre-made electrodes template. For each analyte, two detection zones were used. The carbon working electrode was coated with chitosan-BSA (and enzymes for glucose and uric acid). To detect glucose and uric acid, enzymatic reactions were employed. These reactions involve enzyme-catalyzed redox reactions of the analytes and produce free electrons for electrochemical measurement. Calibration curves were linear (R² > 0.980) in the range of 0-80 mM for glucose, 0.09–0.9 mM for dopamine, and 0–50 mM for uric acid, respectively. Blood samples were successfully analyzed by the proposed method. }, journal = {International Journal of Mechanical and Mechatronics Engineering}, volume = {11}, number = {11}, year = {2017}, pages = {1781 - 1784}, ee = {https://publications.waset.org/pdf/10008143}, url = {https://publications.waset.org/vol/131}, bibsource = {https://publications.waset.org/}, issn = {eISSN: 1307-6892}, publisher = {World Academy of Science, Engineering and Technology}, index = {Open Science Index 131, 2017}, }