TY - JFULL AU - Sahira N. Muslim and Alaa N. Mohammed and Saba Saadoon Khazaal and Batool Kadham Salman and Israa M. S. AL-Kadmy and Sraa N. Muslim and Ahmed S. Dwaish and Sawsan Mohammed Kareem and Sarah N. Aziz and Ruaa A. Jasim PY - 2017/8/ TI - Improvement of Antibacterial Activity for Ceftazidime by Partially Purified Tannase from Penicillium expansum T2 - International Journal of Biotechnology and Bioengineering SP - 540 EP - 545 VL - 11 SN - 1307-6892 UR - https://publications.waset.org/pdf/10007789 PU - World Academy of Science, Engineering and Technology NX - Open Science Index 127, 2017 N2 - Tannase has wide applications in food, beverage, brewing, cosmetics and chemical industries and one of the major applications of tannase is the production of gallic acid. Gallic acid is used for manufacturing of trimethoprim. In the present study, a local fungal strain of Penicillium expansum A4 isolated from spoilt apple samples gave the highest production level of tannase. Tannase was partially purified with a recovery yield of 92.52% and 6.32 fold of purification by precipitation using ammonium sulfate at 50% saturation. Tannase led to increased antimicrobial activity of ceftazidime against Pseudomonas aeruginosa and S. aureus and had a synergism effect at low concentrations of ceftazidime, and thus, tannase may be a useful adjuvant agent for the treatment of many bacterial infections in combination with ceftazidime. ER -