WASET
	@article{(Open Science Index):https://publications.waset.org/pdf/10007365,
	  title     = {Displaying of GnRH Peptides on Bacteriophage T7 and Its Immunogenicity in Mice Model},
	  author    = {Hai Xu and  Yiwei Wang and  Xi Bao and  Bihua Deng and  Pengcheng Li and  Yu Lu},
	  country	= {},
	  institution	= {},
	  abstract     = {T7 phage could be used as a perfect vector for peptides expression and haptens presentation. T7-3GnRH recombinant phage was constructed by inserting three copies of Gonadotrophin Releasing Hormone (GnRH) gene into the multiple cloning site of T7 Select 415-1b phage genome. The positive T7-3GnRH phage was selected by using polymerase chain reaction amplification, and the p10B-3GnRH fusion protein was verified by SDS-PAGE and Western-blotting assay. T7-3GnRH vaccine was made and immunized with 1010 pfu in 0.2 ml per dose in mice. Blood samples were collected at an interval in weeks, and anti-GnRH antibody and testosterone concentrations were detected by ELISA and radioimmunoassay, respectively. The results show that T7-3GnRH phage particles confer a high immunogenicity to the GnRH-derived epitope. Moreover, the T7-3GnRH vaccine induced higher level of anti-GnRH antibody than ImproVac®. However, the testosterone concentrations in both immunized groups were at a similar level, and the testis developments were significantly inhibited compared to controls. These findings demonstrated that the anti-GnRH antibody could neutralize the endogenous GnRH to down regulate testosterone level and limit testis development, highlighting the potential value of T7-3GnRH in the immunocastration vaccine research.
},
	    journal   = {International Journal of Bioengineering and Life Sciences},
	  volume    = {11},
	  number    = {6},
	  year      = {2017},
	  pages     = {455 - 458},
	  ee        = {https://publications.waset.org/pdf/10007365},
	  url   	= {https://publications.waset.org/vol/126},
	  bibsource = {https://publications.waset.org/},
	  issn  	= {eISSN: 1307-6892},
	  publisher = {World Academy of Science, Engineering and Technology},
	  index 	= {Open Science Index 126, 2017},
	}