Wet Polymeric Precipitation Synthesis for Monophasic Tricalcium Phosphate
Tricalcium phosphate (β-Ca3(PO4)2, β-TCP) powders were synthesized using wet polymeric precipitation method for the first time to our best knowledge. The results of X-ray diffraction analysis showed the formation of almost single a Ca-deficient hydroxyapatite (CDHA) phase of a poor crystallinity already at room temperature. With continuously increasing the calcination temperature up to 800 °C, the crystalline β-TCP was obtained as the main phase. It was demonstrated that infrared spectroscopy is very effective method to characterize the formation of β-TCP. The SEM results showed that β-TCP solids were homogeneous having a small particle size distribution. The β-TCP powders consisted of spherical particles varying in size from 100 to 300 nm. Fabricated β-TCP specimens were placed to the bones of the rats and maintained for 1-2 months.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1129944Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 626
 M. Winter, P. Griss, K. de Groot, H. Tagai, G. Heimke, HJ von Dijk, K Sawai. “Comparative histocompatibility testing of seven calcium phosphate ceramics”, Biomater., vol. 2, pp. 159–160, 1981.
 N. Passuti, G. Daculsi, J.M. Rogez, S. Martin, J.V. “Bainvel. Macroporous calcium phosphate ceramic performance in human spine fusion”. Clin. Orthop. Relat. Res., vol. 248, pp. 169-176, 1989.
 S-I. Roohani-Esfahani, Y.J. No, Z.F. Lu, P.Y. Ng, Y.J. Chen, J. Shi, N.J. Pavlos, H. Zreiqat. “A bioceramic with enhanced osteogenic properties to regulate the function of osteoblastic and osteocalastic cells for bone tissue regeneration”, Biomed. Mater., vol. 11, 2016, p.035018.
 M. Bohner, “Calcium orthophosphates in medicine: from ceramics to calcium phosphate cements”. Injury, vol. 31, pp. SD37-SD47, 2000.
 S.V. Dorozhkin, M. Epple. “Biological and medical significance of calcium phosphates”, Angew. Chem. Int. Ed. Engl., vol. 41, pp. 3130-3146, 2002.
 T. Takahata, T. Okihara, Y. Yoshida, K. Yoshihara, Y. Shiozaki, A. Yoshida, K. Yamane, N. Watanabe, M. Yoshimura, M. Nakamura, M. Irie, B. Van Meerbeek, M. Tanaka, T. Ozaki, A. Matsukawa. “Bone engineering by phosphorylated-pullulan and beta-TCP composite”. Biomed. Mater., vol. 10, p. 065009, 2015.
 G. Tozzi, A. De Mori, A. Oliveira, M. Roldo. “Composite hydrogels for bone regeneration” Mater. , vol. 9, UNSP 267, 2016.
 J. Kolmas, S. Krukowski, A. Laskus, M. Jurkitewicz. “Synthetic hydroxyapatite in pharmaceutical applications,” Ceram. Int., vol. 42, pp. 2472-2487, 2016.
 S. Utech, A.R. Boccaccini. “A review of hydrogel-based composites for biomedical applications: enhancement of hydrogel properties by addition of rigid inorganic fillers,” J. Mater. Sci., vol. 51, pp. 271-310, 2016.
 R.Z. Le Geros, “Properties of osteoconductive biomaterials: calcium phosphates”. Clin. Orthop., vol. 395 pp. 81-98, 2002.
 D.S Metsger, T.D. Driskell, J.R. Paulsrud. “Tricalcium phosphate ceramic--a resorbable bone implant: review and current status”, J. Am. Dent. Assoc., vol. 105, pp. 1035-1038, 1982.
 J.X. Lu, A. Gallur, B. Flautre, K. Anselme, M. Descamps, B. Thierry, P. Hardouin. “Comparative study of tissue reactions to calcium phosphate ceramics among cancellous, cortical, and medullar bone sites in rabbits”, J. Biomed. Mater. Res., vol. 42, pp. 357–367, 1998.
 M. Bohner, G.H. Lenthe, S. Gruenenfelder, W. Hirsiger, R. Evison, R. Mueller., “Synthesis and characterization of porous beta-tricalcium phosphate blocks”, Biomater., vol. 26, pp. 6099–6105, 2005.
 DSH Lee, Y. Pai, S. Chang, D.H. Kim. “Microstructure, physical properties, and bone regeneration effect of the nano-sized β-tricalcium phosphate granules”, Mater. Sci. Eng. C., vol. 58, pp. 971–976, 2016.
 K. Ishikawa, N. Koga, K. Tsuru, I. Takahashi. “Fabrication of interconnected porous calcite by bridging calcite granules with dicalcium phosphate dihydrate and their histological evaluation”, J. Biomed. Mater. Res. Part A., vol. 104, pp. 652-658, 2016.
 Y. Pan, J.L. Huang, C.Y. Shao. “Preparation of β-TCP with high thermal stability by solid reaction route”, J. Mater. Sci., vol. 38, pp. 1049–1056, 2003.
 J.S. Cho, D.S. Jung, J.M. Han, Y.C. Kang. “Nano-sized α and β-TCP powders prepared by high temperature flame spray pyrolysis”, Mater. Sci. Eng. C., vol. 29, pp. 1288–1292, 2009.
 K.P. Sanosh, M.C. Chu, A. Balakrishnan, T.N. Kim, S.J. Cho, “Sol–gel synthesis of pure nano sized b-tricalcium phosphate crystalline powders”, Curr. Appl. Phys., vol. 10, pp. 68–71, 2010.
 S.C. Liou, S.Y. Chen. “Transformation mechanism of different chemically precipitated apatitic precursors into β-tricalcium phosphate upon calcinations”, Biomater., vol. 23, pp. 4541–4547, 2002.
 D.R.R. Lazar, S.M. Cunha, V. Ussui, E. Fancio, N.B. de Lima, A.H.A. Bressiani. “Effect of calcination conditions on phase formation of calcium phosphates ceramics synthesized by homogeneous precipitation”, Mater. Sci. Forum., vol. 530, pp.612–617, 2006.
 M. Akao, H. Aoki, K. Kato, A. Sato. “Dence polycrystalline b-tricalcium phosphate for prosthetic applications”, J. Mater. Sci., vol. 17 343–346, 1982.
 A. Destainville, E. Champion, D. Bernache-Assollant, E. Laborde. “Synthesis, characterization and thermal behavior of apatitic tricalcium phosphate”, Mater. Chem. Phys., vol. 80, pp. 269–277, 2003.
 S.C. Liou, S.Y. Chen, H.Y. Lee, J.S. Bow. “Structural characterization of nano-sized calcium deficient apatite powders”, Biomater., vol. 25, pp. 189–196, 2004.
 B. Dickens, L.W. Schroeder, W.E. Brown. “Crystallographic studies of the role of Mg as a stabilizing impurity in β-Ca3(PO4)2. The crystal structure of pure β-Ca3(PO4)2”, J. Solid State Chem., vol. 10, pp. 232–248,1974.
 R. Jenkins, R.L. Snyder. Chemical Analysis: Introduction to X-ray Powder Diffractometry, Wiley, New York (1996) p. 90.
 I. Bogdanoviciene, M. Cepenko, R. Traksmaa, A. Kareiva, K. Tõnsuaadu. “Formation of Ca-Zn-Na phosphate bioceramic material in thermal processing of EDTA sol-gel precursor”, J. Therm. Anal. Calorim., vol. 121, pp. 107-114, 2015.
 E. Garskaite, L. Alinauskas, M. Drienovsky, J. Krajcovic, R. Cicka, M. Palcut, L. Jonusauskas, M. Malinauskas, Z. Stankeviciute, A. Kareiva. “Fabrication of composite of nanocrystalline carbonated hydroxyapatite (cHAP) with polylactic acid (PLA) and its surface topographical structuring with direct laser writing (DLW)”, RSC Adv., vol.6, ppt. 72733-72743, 2016.
 J. Trinkunaite-Felsen, Z. Stankeviciute, J.C. Yang, T.C.K. Yang, A. Beganskiene, A. Kareiva. “Calcium hydroxyapatite/whitlockite obtained from dairy products: simple, environmentally benign and green preparation technology”, Ceram. Int., vol. 40, pp. 12717-12722, 2014.
 A. Gatelyte, D. Jasaitis, A. Beganskiene, A Kareiva. “Sol-gel synthesis and characterization of selected transition metal nano-ferrites. Materials Science (Medžiagotyra)”, vol. 17, pp. 302-307, 2011.
 S. Hosseini, H. Naderi-Manesh, H. Vali, S. Faghihi. “Improved surface bioactivity of stainless steel substrates using osyeocalcin mimetic peptide”, Mater. Chem. Phys., vol. 143, pp. 1364-1371, 2014.
 M.Koizhaiganova, I. Yasa, G. Gulumser. “Characterization and antimicrobial activity of silver doped hydroxyapatite obtained by the microwave method. Materials Science (Medžiagotyra)”, vol. 22, pp. 403-408, 2016.