@article{(Open Science Index):https://publications.waset.org/pdf/10005743,
	  title     = {In vitro Effects of Amygdalin on the Functional Competence of Rabbit Spermatozoa},
	  author    = {Marek Halenár and  Eva Tvrdá and  Tomáš Slanina and  Ľubomír Ondruška and  Eduard Kolesár and  Peter Massányi and  Adriana Kolesárová },
	  country	= {},
	  institution	= {},
	  abstract     = {The present in vitro study was designed to reveal whether amygdalin (AMG) is able to cause changes to the motility, viability and mitochondrial activity of rabbit spermatozoa. New Zealand White rabbits (n = 10) aged four months were used in the study. Semen samples were collected from each animal and used for the in vitro incubation. The samples were divided into five equal parts and diluted with saline supplemented with 0, 0.5, 1, 2.5 and 5 mg/mL AMG. At times 0h, 3h and 5h spermatozoa motion parameters were assessed using the SpermVision™ computer-aided sperm analysis (CASA) system, cell viability was examined with the metabolic activity (MTT) assay, and the eosin-nigrosin staining technique was used to evaluate the viability of rabbit spermatozoa. All AMG concentrations exhibited stimulating effects on the spermatozoa activity, as shown by a significant preservation of the motility (P<0.05 with respect to 0.5 mg/mL and 1 mg/mL AMG; Time 5 h) and mitochondrial activity (P< 0.05 in case of 0.5 mg/mL AMG; P< 0.01 in case of 1 mg/mL AMG; P < 0.001 with respect to 2.5 mg/mL and 5 mg/mL AMG; Time 5 h). None of the AMG doses supplemented had any significant impact of the spermatozoa viability. In conclusion, the data revealed that short-term co-incubation of spermatozoa with AMG may result in a higher preservation of the sperm structural integrity and functional activity.},
	    journal   = {International Journal of Animal and Veterinary Sciences},
	  volume    = {10},
	  number    = {11},
	  year      = {2016},
	  pages     = {712 - 716},
	  ee        = {https://publications.waset.org/pdf/10005743},
	  url   	= {https://publications.waset.org/vol/119},
	  bibsource = {https://publications.waset.org/},
	  issn  	= {eISSN: 1307-6892},
	  publisher = {World Academy of Science, Engineering and Technology},
	  index 	= {Open Science Index 119, 2016},
	}