TY - JFULL AU - Li Li and Li-Ru Xia and He-Ye Wang and Xiao-Dong Bi PY - 2016/7/ TI - Fabrication of Immune-Affinity Monolithic Array for Detection of α-Fetoprotein and Carcinoembryonic Antigen T2 - International Journal of Biotechnology and Bioengineering SP - 383 EP - 388 VL - 10 SN - 1307-6892 UR - https://publications.waset.org/pdf/10005481 PU - World Academy of Science, Engineering and Technology NX - Open Science Index 114, 2016 N2 - In this paper, we presented a highly sensitive immune-affinity monolithic array for detection of α-fetoprotein (AFP) and carcinoembryonic antigen (CEA). Firstly, the epoxy functionalized monolith arrays were fabricated using UV initiated copolymerization method. Scanning electron microscopy (SEM) image showed that the poly(BABEA-co-GMA) monolith exhibited a well-controlled skeletal and well-distributed porous structure. Then, AFP and CEA immune-affinity monolithic arrays were prepared by immobilization of AFP and CEA antibodies on epoxy functionalized monolith arrays. With a non-competitive immune response format, the presented AFP and CEA immune-affinity arrays were demonstrated as an inexpensive, flexible, homogeneous and stable array for detection of AFP and CEA. ER -