Fabrication of Immune-Affinity Monolithic Array for Detection of α-Fetoprotein and Carcinoembryonic Antigen

Authors: Li Li, Li-Ru Xia, He-Ye Wang, Xiao-Dong Bi

Abstract:

In this paper, we presented a highly sensitive immune-affinity monolithic array for detection of α-fetoprotein (AFP) and carcinoembryonic antigen (CEA). Firstly, the epoxy functionalized monolith arrays were fabricated using UV initiated copolymerization method. Scanning electron microscopy (SEM) image showed that the poly(BABEA-co-GMA) monolith exhibited a well-controlled skeletal and well-distributed porous structure. Then, AFP and CEA immune-affinity monolithic arrays were prepared by immobilization of AFP and CEA antibodies on epoxy functionalized monolith arrays. With a non-competitive immune response format, the presented AFP and CEA immune-affinity arrays were demonstrated as an inexpensive, flexible, homogeneous and stable array for detection of AFP and CEA.

Keywords: Chemiluminescent detection, immune-affinity, monolithic copolymer array, UV-initiated copolymerization.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1126744

References:

[1] B. Joshua, O. Miguel, K. Yordan, A.B. Hugh, R. Avraham, “Lensless CCD-based fluorometer using a micromachined optical Söller collimator”, Lab Chip, vol. 11, pp. 941-949, 2011.
[2] S. Parween, P. Nahar, “Image-based ELISA on an activated polypropylene microtest plate—A spectrophotometer-free low cost assay technique”, Biosens. Bioelectron., vol. 48, pp. 287-292, 2013.
[3] M. Mirasoli, M. Guardigli, E. Michelini, A. Roda, “Recent advancements in chemical luminescence-based lab-on-chip and microfluidic platforms for bioanalysis”, J. Pharmaceut. Biomed. Anal., vol. 87, pp. 36-52, 2014.
[4] J. Horak, C. Dincer, H. Bakirci, G. Urban, “A disposable dry film photoresist-based microcapillary immunosensor chip for rapid detection of Epstein–Barr virus infection”, Sensor. Actuat. B Chem., vol. 191, pp. 813-820, 2014.
[5] L. Li, Y. Lu, Z.J. Bie, H.Y. Chen, Z. Liu, “Photolithographic Boronate Affinity Molecular Imprinting: A General and Facile Approach for Glycoprotein Imprinting”, Angew. Chem. Int. Ed., vol. 52, pp. 7451-7454, 2013.
[6] C. Cafer, P. F. Jerome, P. L. James, C. Perihan, “Development of a micro-total analysis system (μ-TAS) for the determination of catecholamines”, Anal. Bioanal. Chem., vol. 398, pp. 1909-1917, 2010.
[7] Y.C. Liu, Y. Lu, Z. Liu, “Restricted access boronate affinity porous monolith as a protein A mimetic for the specific capture of immunoglobulin G”, Chem. Sci., vol. 3, pp. 1467-1471, 2012.
[8] L. Li, X. D. Bi, M. Feng, J. T. Zhu, “UV-initiated copolymerization route for facile fabrication of epoxy-functionalized micro-zone plates”, J. Applied Polymer Science, vol.131, pp. 39787-39793, 2014.
[9] L. Li, X. D. Bi, J. Z. Yu, C. L. Ren, Z. Liu, “A new soft lithographic route for the facile fabrication of hydrophilic sandwich microchips”, Electrophoresis, vol. 33, pp. 2591–2597, 2012.
[10] L. Li, Z.H. He, W.W. Wang, Y.M. Fan, X.P. Mao, D.H. Chen, C. Chen, “Synthesis of amino-functionalized epoxy acrylate film by magnetic-filtered plasma stream”, Prog. Org. Coat., vol. 56, pp. 126-130, 2006.
[11] L. Li, L. R. Xia, H. Y. Wang, X. D. Bi, “New method for fabricating an α-fetoprotein affinity monolithic polymer array”, J. Applied Polymer Science, vol. 132, pp. 41792-41796, 2015.
[12] Z. F. Fu, C. Hao, X. Q. Fei, H. X. Ju, “Flow-injection chemiluminescent immunoassay for α-fetoprotein based on epoxysilane modified glass microbeads”, Journal of Immunological Methods, vol. 312, pp. 61-67, 2006.