Extraction of Squalene from Lebanese Olive Oil

Authors: Henri El Zakhem, Christina Romanos, Charlie Bakhos, Hassan Chahal, Jessica Koura

Abstract:

Squalene is a valuable component of the oil composed of 30 carbon atoms and is mainly used for cosmetic materials. The main concern of this article is to study the Squalene composition in the Lebanese olive oil and to compare it with foreign oil results. To our knowledge, extraction of Squalene from the Lebanese olive oil has not been conducted before. Three different techniques were studied and experiments were performed on three brands of olive oil, Al Wadi Al Akhdar, Virgo Bio and Boulos. The techniques performed are the Fractional Crystallization, the Soxhlet and the Esterification. By comparing the results, it is found that the Lebanese oil contains squalene and Soxhlet method is the most effective between the three methods extracting about 6.5E-04 grams of Squalene per grams of olive oil.

Keywords: Squalene, extraction, crystallization, Soxhlet.‎

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1111887

References:

[1] B. Raina, “Olives”, Encyclopedia of food sciences and nutrition, 2, 2003 4260 - 4267.
[2] G. Reglero, G. Torello, & C. Torres. “Extraction and enzymatic modification of functional lipids from soybean oil deodorizer distillate,” Retreived from http://www.intechopen.com/books/recent-trends-for-enhancing-the-diversity-and-quality-of-soybean-products/extraction-and-enzymatic-modification-of-functional-lipids-from-soybean-oil-deodorizer-distillate, 2011.
[3] C. Gutiérrez, A. Siles, F. Chica, & A. Martin. “Kinetics of biofuel generation from deodorizer distillates derived from the physical refining of olive oil and squalene recovery,” Biomass & Bioenergy, 62, 2014, 93-99. Doi: 10.1016/j.biombioe.2014.01.010.
[4] A. Raminez-Torrres, C. Gabas, & C. Barranquero. “Squalene: Current knowledge and potential,” New York: Nova Biomedical Books, 2011.
[5] M. Tsimidoum. “Olives and olive oil in health and disease prevention (V. R. Preedy, & R. R. Watson, Eds),” Academic Press, New York, 2010.
[6] B. Wetherbee, & P. Nichols. “Lipid composition of the liver oil of deep-sea sharks from the chatham rise,” Comparative Biochemistry & Physiology Part B, New Zealand., 125, 1999, 511-521.
[7] P. Kraujalis & P. Venskutonis. “Supercritical carbon dioxide extraction of squalene and tocopherols fromamaranth and assessment of extracts antioxidant activity,” The Journal of Supercritical Fluids, 80, 2013, 79-85.
[8] P. Dugic, M. Jotanovic, Z. Lepojevic. “Supercritical fluid extraction with carbon dioxide at differential pressures,” Original Scientific Papers, 2011, 84-87. Doi:10.5767/anurs.cmat.110201.en.084M.
[9] S. Gunawan, N. Kasim, & Y. Ju. “Separation and purification of squalene from soybean oil deodorizer distillate,” Separation and Purification Technology, 60, 2007, 128–135.
[10] N. Nenadis, & M. Tsimidou. “Determination of squalene in olive oil using fractional crystallization for sample preparation,” JAOCS, 79 (3), 2002, 257-259.
[11] M. Tsimidou, G. Blekas, & D. Boskou. “Olive Oil,” In Encyclopedia of food sciences and nutrition. Retrieved from http://www.sciencedirect.com/science/article/ pii/B012227055X01347X?np=y, 2003.
[12] D. Grigoriadoua, A. Androulaki, E. Psomiadoub, & M. Tsimidou. “Solid phase extraction in the analysis of squalene and tocopherols in olive oil,” Food Chemistry, 105, 2006, 675–680.
[13] A. Fernández-Cuesta, L. León, L. Velascoa, & R. De la Rosa. "Changes in squalene and sterols associated with olive maturation,” Food Research International, 54, 2013, 1885–1889.