@article{(Open Science Index):https://publications.waset.org/pdf/10003684,
	  title     = {Influence of AgNO3 Treatment on the Flavonolignan Production in Cell Suspension Culture of Silybum marianum (L.) Gaertn},
	  author    = {Anna Vildová and  H. Hendrychová and  J. Kubeš and  L. Tůmová},
	  country	= {},
	  institution	= {},
	  abstract     = {The abiotic elicitation is one of the methods for
increasing the secondary metabolites production in plant tissue
cultures and it seems to be more effective than traditional strategies.
This study verified the use of silver nitrate as elicitor to enhance
flavonolignans and flavonoid taxifolin production in suspension
culture of Sylibum marianum (L.) Gaertn. Silver nitrate in various
concentrations (5.887.10-3 mol/L, 5.887.10-4 mol/L, 5.887.10-5
mol/L) was used as elicitor. The content of secondary metabolites in
cell suspension cultures was determined by high performance liquid
chromatography. The samples were taken after 6, 12, 24, 48, 72 and
168 hours of treatment. The highest content of taxifolin production
(2.2 mg.g-1) in cell suspension culture of Silybum marianum (L.)
Gaertn. was detected after silver nitrate (5.887.10-4 mol/L) treatment
and 72 h application. Flavonolignans such as silybinA, silybin B,
silydianin, silychristin, isosilybin A, isosilybin B were not produced
by cell suspension culture of S. marianum after elicitor treatment.
Our results show that the secondarymetabolites could be released
from S. marianum cells into the nutrient medium by changed
permeability of cell wall.},
	    journal   = {International Journal of Agricultural and Biosystems Engineering},
	  volume    = {8},
	  number    = {8},
	  year      = {2014},
	  pages     = {959 - 962},
	  ee        = {https://publications.waset.org/pdf/10003684},
	  url   	= {https://publications.waset.org/vol/92},
	  bibsource = {https://publications.waset.org/},
	  issn  	= {eISSN: 1307-6892},
	  publisher = {World Academy of Science, Engineering and Technology},
	  index 	= {Open Science Index 92, 2014},
	}