Analysis of DNA from Fired Cartridge Casings
Commenced in January 2007
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Analysis of DNA from Fired Cartridge Casings

Authors: S. Mawlood, L. Dennany, N. Watson, B. Pickard

Abstract:

DNA analysis has been widely accepted as providing valuable evidence concerning the identity of the source of biological traces. Our work has showed that DNA samples can survive on cartridges even after firing. The study also raised the possibility of determining other information such as the age of the donor. Such information may be invaluable in certain cases where spent cartridges from automatic weapons are left behind at the scene of a crime. In spite of the nature of touch evidence and exposure to high chamber temperatures during shooting, we were still capable to retrieve enough DNA for profile typing. In order to estimate age of contributor, DNA methylation levels were analyzed using EpiTect system for retrieved DNA. However, results were not conclusive, due to low amount of input DNA.

Keywords: Age prediction, Fired cartridge, Trace DNA sample.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1108300

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References:


[1] Bocklandt S LW, Sehl ME, Sanchez FJ, Sinsheimer JS, Steve Horvath and Eric Vilain. Epigenetic Predictor of Age. PLoS ONE. 2011; 6(6).
[2] Burgess DJ. Human epigenetics: Showing your age. Nature Reviews Genetics. 2012;14(1):6-.
[3] Van Oorschot R, Ballantyne KN, Mitchell RJ. Forensic trace DNA: a review. Investig genet. 2010;1(1):14.
[4] Meissner C, Ritz-Timme S. Molecular pathology and age estimation. Forensic science international. 2010;203(1–3):34-43.
[5] Zubakov D, Liu F, van Zelm MC, Vermeulen J, Oostra BA, van Duijn CM, et al. Estimating human age from T-cell DNA rearrangements. Current Biology. 2010;20(22):R970-R1.
[6] Evett I, Pinchin R, Buffery C. An investigation of the feasibility of inferring ethnic origin from DNA profiles. Journal of the Forensic Science Society. 1992;32(4):301-6.
[7] Locard E. The analysis of dust traces. Part I. The American Journal of Police Science. 1930;1(3):276-98.
[8] Wickenheiser RA. Trace DNA: a review, discussion of theory, and application of the transfer of trace quantities of DNA through skin contact. Journal of forensic sciences. 2002;47(3):442.
[9] Williamson AL. Touch DNA: Forensic Collection and Application to Investigations. J Assoc Crime Scene Reconstr. 2012;18(1).
[10] Mawlood SK, Alrowaithi M, Watson N. Advantage of ForensiX Swabs in Retrieving and Preserving Biological Fluids. J Forensic Sci, May 2015, Vol. 60, No. 3. doi: 10.1111/1556-4029.12704.
[11] Naseem M. DNA Extraction from Casework Samples using the Applied Biosystems® AutoMate Express™ Forensic DNA Extraction System 2011 (cited 214 10,15). Available from: http://www.appliedbiosystems.com/ForensicNews-Past
[12] Liu JY, Zhong C, Holt A, Lagace R, Harrold M, Dixon AB, et al. AutoMate Express™ Forensic DNA Extraction System for the Extraction of Genomic DNA from Biological Samples*. Journal of forensic sciences. 2012;57(4):1022-30.
[13] Montpetit SA, Fitch IT, O’Donnell PT. A simple automated instrument for DNA extraction in forensic casework. J Forensic Sci2005. p. 555-63.
[14] Butler JM, Shen Y, McCord BR. The development of reduced size STR amplicons as tools for analysis of degraded DNA. Journal of forensic sciences. 2003; 48(5):1054-64.
[15] Biosystems A. AmpFLSTR® MiniFiler™ PCR Amplification Kit User Guide. July 2014.
[16] Polley D, Mickiewicz P, Vaughn M, Miller T, Warburton R, Komonski D, et al. An investigation of DNA recovery from firearms and cartridge cases. Journal-Canadian Society of Forensic Science. 2006;39(4):217.
[17] Ryan SR. Successful DNA profiling from fired casings, bullets and cartridges June 2012; vol.1, Issue 4. Available from: http://www.ryanforensicdna.com/yahoo_site_admin/assets/docs/June_N ewsletter.163101437.pdf
[18] Dominick A, Welch L, Nic Daeid N, Bleay SM. Is there a relationship between fingerprint donation and DNA shedding? Journal of Forensic Identification. 2009;59(2):133.
[19] Ravreby M. Analysis of long-range bullet entrance holes by atomic absorption spectrophotometry and scanning electron microscopy. Journal of forensic sciences. 1982;27(1):92-112.
[20] Quinones I, Daniel B. Cell free DNA as a component of forensic evidence recovered from touched surfaces. Forensic Science International: Genetics. 2012;6(1):26-30.
[21] Koons RD, Grant DM. Compositional variation in bullet lead manufacture. Journal of forensic sciences. 2002;47(5):950-8.
[22] Sutherland E, Coe L, Raleigh EA. McrBC: a multisubunit GTPdependent restriction endonuclease. Journal of molecular biology. 1992;225(2):327-48.
[23] Stewart F, Raleigh E. Dependence of McrBC cleavage on distance between recognition elements. Biological chemistry. 1997;379(4-5):611- 6.
[24] QIAGEN. EpiTect Methyl II PCR Assay Handbook. www.qiagen.com; 2012.
[25] Horsman-Hall KM, Orihuela Y, Karczynski SL, Davis AL, Ban JD, Greenspoon SA. Development of STR profiles from firearms and fired cartridge cases. Forensic Science International: Genetics. 2009;3(4):242- 50.
[26] Nunn S. Touch DNA Collection Versus Firearm Fingerprinting: Comparing Evidence Production and Identification Outcomes. Journal of forensic sciences. 2013.
[27] Branch L. Generating forensic DNA profiles from'contact'DNA on cartridge cases and gun grips 2011.