@article{(Open Science Index):https://publications.waset.org/pdf/10001550,
	  title     = {Conformation Prediction of Human Plasmin and Docking on Gold Nanoparticle},
	  author    = {Wen-Shyong Tzou and  Chih-Ching Huang and  Chin-Hwa Hu and  Ying-Tsang Lo and  Tun-Wen Pai and  Chia-Yin Chiang and  Chung-Hao Li and  Hong-Jyuan Jian},
	  country	= {},
	  institution	= {},
	  abstract     = {Plasmin plays an important role in the human
circulatory system owing to its catalytic ability of fibrinolysis. The
immediate injection of plasmin in patients of strokes has intrigued
many scientists to design vectors that can transport plasmin to the
desired location in human body. Here we predict the structure of
human plasmin and investigate the interaction of plasmin with the
gold-nanoparticle.
Because the crystal structure of plasminogen has been solved, we
deleted N-terminal domain (Pan-apple domain) of plasminogen and
generate a mimic of the active form of this enzyme (plasmin). We
conducted a simulated annealing process on plasmin and discovered a
very large conformation occurs. Kringle domains 1, 4 and 5 had been
observed to leave its original location relative to the main body of the
enzyme and the original doughnut shape of this enzyme has been
transformed to a V-shaped by opening its two arms. This observation
of conformational change is consistent with the experimental results of
neutron scattering and centrifugation.
We subsequently docked the plasmin on the simulated gold surface
to predict their interaction. The V-shaped plasmin could utilize its
Kringle domain and catalytic domain to contact the gold surface.
Our findings not only reveal the flexibility of plasmin structure but
also provide a guide for the design of a plasmin-gold nanoparticle.},
	    journal   = {International Journal of Biotechnology and Bioengineering},
	  volume    = {9},
	  number    = {6},
	  year      = {2015},
	  pages     = {488 - 491},
	  ee        = {https://publications.waset.org/pdf/10001550},
	  url   	= {https://publications.waset.org/vol/102},
	  bibsource = {https://publications.waset.org/},
	  issn  	= {eISSN: 1307-6892},
	  publisher = {World Academy of Science, Engineering and Technology},
	  index 	= {Open Science Index 102, 2015},
	}