%0 Journal Article
	%A Siobhan O’Shea and  Sangeetha Vijaysri Nair and  Hee Cheol Kim and  Charles Thomas Nugent and  Cheuk Yan William Tong and  Sam Douthwaite and  Andrew Worlock
	%D 2015
	%J International Journal of Medical and Health Sciences
	%B World Academy of Science, Engineering and Technology
	%I Open Science Index 101, 2015
	%T Performance of the Aptima® HIV-1 Quant Dx Assay on the Panther System
	%U https://publications.waset.org/pdf/10001321
	%V 101
	%X The Aptima® HIV-1 Quant Dx Assay is a fully
automated assay on the Panther system. It is based on Transcription-
Mediated Amplification and real time detection technologies. This
assay is intended for monitoring HIV-1 viral load in plasma
specimens and for the detection of HIV-1 in plasma and serum
specimens.
Nine-hundred and seventy nine specimens selected at random
from routine testing at St Thomas’ Hospital, London were
anonymised and used to compare the performance of the Aptima
HIV-1 Quant Dx assay and Roche COBAS® AmpliPrep/COBAS®
TaqMan® HIV-1 Test, v2.0. Two-hundred and thirty four specimens
gave quantitative HIV-1 viral load results in both assays. The
quantitative results reported by the Aptima Assay were comparable to
those reported by the Roche COBAS AmpliPrep/COBAS TaqMan
HIV-1 Test, v2.0 with a linear regression slope of 1.04 and an
intercept on -0.097.
The Aptima assay detected HIV-1 in more samples than the
COBAS assay. This was not due to lack of specificity of the Aptima
assay because this assay gave 99.83% specificity on testing plasma
specimens from 600 HIV-1 negative individuals. To understand the
reason for this higher detection rate a side-by-side comparison of low
level panels made from the HIV-1 3rd international standard
(NIBSC10/152) and clinical samples of various subtypes were tested
in both assays. The Aptima assay was more sensitive than the
COBAS assay.
The good sensitivity, specificity and agreement with other
commercial assays make the HIV-1 Quant Dx Assay appropriate for
both viral load monitoring and detection of HIV-1 infections.
	%P 397 - 400